Email this article GENETIC DIVERSITY OF Dendrocalamus asper IN JAVA REVEALED BY RAPD MARKERS
DOI: 10.20886/ijfr.2006.3.1.67-74Abstract
Dendracalamusasper is one of the most widely use and widely distributed bamboo species in Indonesia. A genetic conservation plot has been established to maintain genetic diversity of the species. A total of 115 samples were collected from 12 sites throughout Java. Genetic diversity was revealed using 31 RAPD primers producing 64 polymorphic bands. A total of 81 different multi-locus genotypes (genets) were identified between 115 samples. TI1e total number of multi-locus genotypes in all populations ranged from 3 to 9 (mean 6.5), indicating that all populations consisted of multiple genotypes. Mean genetic diversity was 0.04, (ranging from the lowest 0.009 in Kuningan population to the highest 0.144 in Purwokerto population). The Simpson's diversity index indicated that the most population composed of unique genotype. Geographical differences did not correlate with genetic cluster.
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Dransfield, S. and E.A. Widjaja (Eds.). 1995. Bamboos. Plant Resources of South-East Asia. No. 7.Bogor,Indonesia.
Ellstrand, N.C. and M.L. Roose. 1987. Patterns of genotypic diversity in clonal plant species. American Journal of Botany 74: 123-131.
Ellstrand, N.C. and D.R. Elam. 1993. Population genetic consequences of small population size:implications for plant conservation. Annual Review of Ecology and Systematics,
:219-242.
Hamrick,j.L. and MJ.W. Godt. 1989. Allozyme diversity in plant species. In A.H.D. Brown, M.T. Clegg, A.L. Kahler and B.S. Weir (eds.). Plant Population Genetics, Breeding and Genetic Resources. Sinaeur Associates, Sunderland, Massachusetts. Pp: 43-63.
Hamrick, J.L., MJW. Godt and SL. Sherman-Broyles. 1992. Factor influencing levels of genetic diversity in woody plant species.New Forests 6: 95-124.
Jover, M.A., L. Castillo-Agudo, M. Garcia-Carrascosa, and J. Segura. 2003. Random amplified polymorphic DNA assessment of diversity in western Mediterranean populations of the seagrass Posidoniaoceanica. American Journal of Botany 90(3): 364-369.
Kreher, S.A., S.A. Fore and B.S. Collins. 2000. Genetic variation within and among patches of the clonal species, 'Vaeiniam stamineio»L. Molecular Ecology 9: 1247-1252.
Lee, N.M. and M.G. Chung. 1999. High levels of genetic variation in Korean populations of
Sasamorpha borea/is (Poaceae). Bot. Bull. A cad. Sin. 40:311-317.
S.L., R. Wickneswari, M.C. Mahani and A.H. Zakri. 2000. Genetic diversity of a tropical tree species, Sborea leprosHla Miq, (Dipterocarpaceae), in Malaysia: Implications for conservation of genetic resources and tree improvement. Biotropica 32(2): 213-224.
D.A. and J.L. Hamrick. 1990. Local genetic and clonal structure in the tropical terrestrial bromeliad, .Aecbemea magdalenae. American Journal of Botany 77: 86-89.
M.G. and W.F. Thompson. 1980, Rapid isolation of high molecular weight plants Nucleic Acid Research. 8 : 4321-4325.
Genetic distance between populations. American Naturalist 106: 283-292.
_______. 1973. Analysis of gene diversity in sub-divided populations. Proceedings of the National Academyof Science 70: 3321-3323.
_____ . 1977. F-statistics and analysis of gene diversityin sub-divided populations. Annual of Human Genetics 41: 225-233.
Pielou, C.E. 1969. An introduction to mathematical ecology. Wiley-Interscience, New York, 327p.
Sipes, S.D. and P.G. Wolf.1997. Clonal structure and patterns of allozyme diversity in the rare endemic Cycladenia humilis var. jonesii (Apocynaceae). American Journal of Botany 84: 401-409.
Shiraishi, S. and A. Watanabe. 1995. Identification of chloroplast genome between densijlora SIEB et ZUCC and P thu11bergii PARL based on the polymorphism in gene.Journal] apan Forest Society 77: 429-436.
Williams, J.G.K., A.R. Kubelik, K.J. Livak, J.A. Rafalski and S.V. Tingey. 1990. polymorphisms amplified by arbitrary primers are useful as genetic markers. Acids Research 18: 6531-6535.
Yeh, EC., RC. Yang and T. Boyle. 1993. POPGENE Versionl.3.1. Microsoft Windows-based Freeware for Population Genetic Analysis. Universityof Alberta, Canada.
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