AN ATTEMPT TO CONSERVE A VULNERABLE TREE SPECIES OF Santalum album L. THROUGH MICROPROPAGATION

Autor(s): Manikandan Gurusamy, Karolinsobina Charlie, Kalaimathi Palanisamy, Divya Chelladurai, David R Katerere
DOI: 10.59465/ijfr.2024.11.1.81-89

Abstract

A rare kind of  tropical plant in the Santalaceae family is Santalum album. The active  ingredient  in  S. album,  santalol,  is  also  referred  to  as  sandalwood  oil  and  is  highly  prized  in  the fragrance business for its fixative qualities and pleasant, enduring scent. Out of  all the species in the genus Santalum,  S. album  has  the  greatest  oil  concentration  (about  6%).  The  wild  plants  are  overharvested  for their wood, which is used to make santalol, as well as for other uses including woodcarving and traditional medicine.  S. album  is  an  easily  hurt  plant.  Thus,  the  creation  of   an  in  vitro  mass  propagation  protocol for  this  valuable  species  is  necessary  in  order  to  generate  homozygous  clones  with  large  yields  for  the establishment  of   sandalwood  plantations.  In  this  study,  a  full-strength  MS  medium  supplemented  with varying concentrations of  BAP and Kn (0.5-2.5 mg/l) was used to cultivate the shoot tip and intermodal portions  of   S. album  that  were  collected  from  the  wild.  The  maximum  shoot  development  (4.50±0.50) occurred at a BAP concentration of  1.5 mg/l. IBA and IAA were added to the rooting medium along with the developing shoots. IBA (2.0 mg/l) had the highest mean number of  roots (4.90±0.25) and root length (5.75±0.47 cm). Shoots that had been successfully rooted were moved to the field to harden. According to the current study, MS medium with 1.5 mg/l of  BAP and 2.0 mg/l of  IBA is an appropriate technique for micropropagating and conserving S. album is fragile tree species.

Keywords

Vulnerable, shoot tip, internode, BAP, IBA, conservation

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DOI: https://doi.org/10.59465/ijfr.2024.11.1.81-89

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