KERAGAMAN GENETIK Taxus Sumatrana DARI POPULASI ALAM DI JAMBI MENGGUNAKAN PENANDA RAPD

Istiana Prihatini; Dodi Frianto; Eka Novriyanti; ILG Nurtjahjaningsih; AYPBC Widyatmoko

doi:10.20886/jpth.2021.15.2.95-104

KERAGAMAN GENETIK Taxus Sumatrana DARI POPULASI ALAM DI JAMBI MENGGUNAKAN PENANDA RAPD

Istiana Prihatini, Dodi Frianto, Eka Novriyanti, ILG Nurtjahjaningsih, AYPBC Widyatmoko

Abstract

The natural population of Taxus sumatrana in several regions in southern Asia including Indonesia has experienced a decline in number. In Sumatra, the population with very few trees was found in Gunung Kerinci and Gunung Tujuh, Kerinci Regency, Jambi. Conservation efforts to protect the extinction of this species are being carried out, one of which is by exploring seeds from natural populations to be planted ex situ. Information on the genetic diversity of Taxus is needed to determine a conservation strategy for this species. This research
was conducted to obtain information on genetic diversity of Taxus sumatrana. Ten RAPD markers were used to analyze the genetic diversity of 44 individuals from two natural populations, namely Gunung Tujuh and Gunung Kerinci. These markers resulted in 104 loci which were used in the RAPD data analysis using GenAlex ver.6.5 program. The results of the data analysis showed that heterozigosity which showed the level of genetic diversity of the populations of the two populations observed was in moderate level and similar, namely 0.33±0.14 (Gunung Tujuh) and 0.35±0.14 (Gunung Kerinci). The genetic distance between the two populations was also in a moderate value (0.097) and the similarities in genetic structure based on the RAPD markers were showing
genetic variety in moderate level (0.908). The results indicate that the two populations, although geographically close have moderate level of genetic structure. Genetic conservation efforts for future Taxus species could increase the number of individuals from both locations and separate them as two distinct populations.

Keywords

cambium DNA; population genetic; genetic conservation

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References

Anonim. (2021). Data WHO: Kanker Sebabkan 230 Ribu Kematian di Indonesia. Www.Gatra.Com. Retrieved from https://www.gatra.com/detail/news/502871/kesehatan/data-who-kanker-sebabkan-230-ribu-kematian-di-indonesia

Cengel, B. (2005). Genetic characterization of Pinus nigra subspecies pallasiana varieties, natural populations (seed stands), seed orchards and plantations. Middle East Technical University.

Collins, D., Mill, R. R., & Moller, M. (2003). Species separation of Taxus baccata, T. canadensis, and T. cuspidata (Taxaceae) and origins of their reputed hybrids inferred from RAPD and cpDNA data. American Journal of Botany, 90(2), 175–182.

Ellstrand, N. C., & Elam, D. R. (1993). Population genetic conseqences of small population size: Implications for plant conservation. Annual Review of Ecology and Systematics, 24, 217–242. https://doi.org/10.1146/annurev.es.24.110193.001245

Farjon. (2013). Taxus baccata, Common Yew. The IUCN Red List of Threatened Species. Retrieved from http://www.conifers.org/ta/Taxus_baccata.php

Farr, K. (2008). Genus-level approach to taxus species, 1–21. Retrieved from www.conabio.gob.mx/institucion/...CS6 Taxus/WG1-CS6.pdf

Frianto, D. (2021). Visi besar dari langkah awal Kampung Taxus. Kabaralam.Com. Retrieved from https://kabaralam.com/inovasi/visi-besar-dari-langkah-awal-kampung-taxus

Frianto, D., & Novriyanti, E. (2016). Pola penyebaran dan potensi kerapatan Taxus sumatrana di Gunung Tujuh, Kabupaten Kerinci, Jambi, 2, 12–15. https://doi.org/10.13057/psnmbi/m020103

Frianto, D., & Novriyanti, E. (2017). Eksplorasi potensi Taxus sumatrana di Gunung Kerinci , Sumatera. In Seminar Nasional Masyarakat Biodiversity Indonesia (Vol. 3, pp. 471–475). https://doi.org/10.13057/psnmbi/m030329

Gusmiaty, Restu, M., Asrianny, & Larekeng, S. (2016). Polimorfisme penanda RAPD untuk analisis keragaman genetik Pinus merkusii di Hutan Pendidikan Unhas. Jurnal Natur Indonesia, 16(2), 47–53. https://doi.org/10.31258/jnat.16.2.47-53

Haryjanto, L. (2016). Dukungan konservasi sumberdaya genetik Cendana (Santalum album Linn) pada program pemuliaan genetik. Seminar Nasional Pendidikan Biologi Dan Saintek II, 258–263.

Iqbal, J., Meilan, R., & Khan, B. (2020). Assessment of risk, extinction, and threats to Himalayan yew in Pakistan. Saudi Journal of Biological Sciences, 27(2), 762–767. https://doi.org/10.1016/j.sjbs.2019.12.033

Kalima, T., & Susilo, A. (2019). Habitat Characteristic of Taxus sumatrana (Miquel) de Laub in The Kerinci Seblat National Park. In IOP Conference Series: Earth and Environmental Science (Vol. 391). https://doi.org/10.1088/1755-1315/391/1/012068

Katsuki, T., & Luscombe, D. (2013). Taxus cuspidata, Japanese Yew. The IUCN Red List of Threatened Species, 1–10. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2013-1.RLTS.T42549A2987373.en

Kemkes RI. (2020). Jenis Kanker ini Rentan Menyerang Manusia. Retrieved October 14, 2021, from https://www.kemkes.go.id/article/view/20011400002/jenis-kanker-ini-rentan-menyerang-manusia.html

Liu, J., Möller, M., Gao, L. M., Zhang, D. Q., & Li, D. Z. (2011). DNA barcoding for the discrimination of Eurasian yews (Taxus L., Taxaceae) and the discovery of cryptic species. Molecular Ecology Resources, 11(1), :89-100. https://doi.org/10.1111/j.1755-0998.2010.02907.x

Malik, S., Cusido, R., & Mirjalili, M. (2011). Review: Production of the anticancer drug taxol in Taxus baccata suspension cultures. Process Biochem, 46, 23–34.

Mohapatra, K. P., Sehgal, R. N., Sharma, R. K., & Mohapatra, T. (2009). Genetic analysis and conservation of endangered medicinal tree species Taxus wallichiana in the Himalayan region. New Forests, 37(2), 109–121. https://doi.org/10.1007/s11056-008-9112-9

Murray, M. G., & Thompson, W. F. (1980). Rapid isolation of high molecular weight plant DNA. Nucleic Acids Research, 8(19), 4321–4325. Retrieved from https://linkinghub.elsevier.com/retrieve/pii/S1874391913002522

Nimasow, G. (2015). Vanishing Taxus baccata L. due to unsustainable exploitation and climate change in West Kameng and Tawang Districts of Arunachal Pradesh. Earth Sciences, 4(3), 11. https://doi.org/10.11648/j.earth.s.2015040301.12

Novriyanti, E., Wahyudi, A., & Frianto, D. (2020). Hindari Kelangkaan, Perlu Pemanfaatan Lestari Cemara Sumatra. Www.Menlhk.Go.Id. Retrieved from https://www.menlhk.go.id/site/single_post/2713

Nurtjahjaningsih, I., Sukartiningsih, Saranti, A., Sulistyawati, P., & Rimbawanto, A. (2017). Kekerabatan genetik anakan alam ulin (Eusideroxylon zwageri TEIJSM. & BINN.) menggunakan penanda Random Amplified Polymorphism DNA. Jurnal Pemuliaan Tanaman Hutan, 11(1), 25–32. https://doi.org/10.20886/jpth.2017.11.1.25-32

Nurtjahjaningsih, I., Widyatmoko, A. Y. P. B. C., & Rimbawanto, A. (2019). Keragaman genetik populasi kayu kuku (Pericopsis mooniana ( Thwaites ) Thwaites ) di hutan Lamedai berdasarkan penanda RAPD. Jurnal Pemuliaan Tanaman Hutan, 13(1), 25–32.

Park, S., An, J., Park, J., Choo, G., Ngondya, I., Ibrahim, R., & Shim, S. (2012). Genetic diversity in six natural populations of Taxus cuspidata from Baekdu trail and Jeju Island in South Korea analyzed by RAPD-DNA markers. Forest Science and Technology, 8(4), 210–214. https://doi.org/10.1080/21580103.2012.750795

Poerba, Y. S., Wawo, A. H., & Yulita, K. S. (2007). Keragaman fenotipe RAPD Santalum album L . di pulau Timor bagian Timur. Berita Biologi, 8(6), 537–546.

Puspitasari, D. N. (2021). Upaya konservasi tumbuhan anti kanker yang terancam punah. Https://Krcibodas.Lipi.Go.Id/.

Rachmat, H. H. (2008). Variasi genetik dan teknik perbanyakan vegetatif Cemara Sumatra (Taxus sumatrana). Institut Pertanian Bogor.

Rachmat, H. H., Subiakto, A., Siregar, I. Z., & Supriyanto, S. (2010). Uji pertimbuhan stek cemara sumatra Taxus sumatrana (Miquel) de Laub. Jurnal Penelitian Hutan Dan Konservasi Alam, 7(3), 289–298. https://doi.org/10.20886/jphka.2010.7.3.289-298

Rimbawanto, A., Widyatmoko, A. Y. P. B. C., & Sulistyawati, P. (2006). Distribusi keragaman genetik populasi Santalum album berdasarkan penanda RAPD. Jurnal Penelitian Hutan Tanaman, 3(3), 175–181.

Schäfer, D., Vincent, H., Fischer, M., & Kempel, A. (2020). The importance of genetic diversity for the translocation of eight threatened plant species into the wild. Global Ecology and Conservation, 24. https://doi.org/10.1016/j.gecco.2020.e01240

Shah, A., Li, D. Z., Gao, L. M., Li, H. T., & Möller, M. (2008). Genetic diversity within and among populations of the endangered species Taxus fuana (Taxaceae) from Pakistan and implications for its conservation. Biochemical Systematics and Ecology, 36(3), 183–193. https://doi.org/10.1016/j.bse.2007.09.012

Shen, Y. C., Hsu, S. M., Lin, Y. S., Cheng, K. C., Chien, C. T., Chou, C. H., & Cheng, Y. B. (2005). Three new taxane diterpenoids from Taxus sumatrana. Chemical and Pharmaceutical Bulletin, 53(7), 808–810. https://doi.org/10.1248/cpb.53.808

Shen, Y. C., Wang, S. S., Pan, Y. L., Lo, K. L., Chakraborty, R., Chien, C. T., … Lin, Y. C. (2002). New taxane diterpenoids from the leaves and twigs of Taxus sumatrana. Journal of Natural Products, 65(12), 1848–1852. https://doi.org/10.1021/np0202273

Shiraishi, S., & Watanabe, A. (1995). Identifikasi of chloroplast genome between Pinus densiflora Sieb et Zucc and P. thumbergii Parl based on the polymorphism in rbct gene. Journal of Japanese Forestry Society, 77, 429–436.

Spector, T., Thomas, P., & Determann, R. (2011). Taxus floridana, Florida Yew. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2011- 2.RLTS.T30965A9584928.en%0ACopyright:

Spjut, R. W. (2007). Taxonomy and nomenclature of Taxus (Taxaceae). Journal of the Botanical Research Institute of Texas, 1(1), 203–289.

Sunandar, A. (2019). Menyelamatkan yang tersisa: Taxus sumatrana, pohon obat yang terancam punah. Http://Aeknauli.Org/. Retrieved from http://aeknauli.org/menyelamatkan-yang-tersisa-taxus-sumatrana-pohon-obat-yang-terancam-punah/

Sunandar, A., Barus, S. P., Kuswanda, W., & Saputra, M. (2019). Vegetation diversity and conservation implications on habitat of Taxus (Taxus sumatrana Miq. de Laub) in Northern Sumatra. In International Conference on Natural Resources and Technology (ICONART2019) (pp. 365–371). https://doi.org/10.5220/0008554603650371

Susilo, A. (2015). Taxus sumatrana: Sebaran, potensi dan strategi konservasi. In Penguatan apresiasi dan kesadaran konservasi jenis kayu lokal Sumatra bernilai tinggi (pp. 30–40). Pekanbaru: Balai Penelitian Teknologi Serat Tanaman Hutan.

Susilowati, A., Rachmat, H. H., Kholibrina, C. R., Hartini, K. S., & Rambe, H. A. (2019). Propagation for conserving endangered taxol producing tree Taxus sumatrana through shoot cuttings technique. In Journal of Physics: Conference Series (Vol. 1282). https://doi.org/10.1088/1742-6596/1282/1/012110

Syarief, I. S. (2021). 19,3 Juta Orang di Dunia Menderita Kanker, Paling Banyak Kanker Payudara. Www.Suarasurabaya.Net. Retrieved from https://www.suarasurabaya.net/kelanakota/2021/193-juta-orang-di-dunia-menderita-kanker-paling-banyak-kanker-payudara/

Thomas, P. (2011). Taxus contorta, West Himalayan Yew. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2011-2.RLTS.T39147A10170545.en

Thomas, P. (2013a). Taxus brevifolia, Pacific Yew. The IUCN Red List of Threatened Species.

Thomas, P. (2013b). Taxus canadensis, Canadian Yew. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2013-1.RLTS.T42547A2986960.en

Thomas, P. (2013c). Taxus globosa, Mesoamerican Yew. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2013-1.RLTS.T30724A2795235.en

Thomas, P., & Farjon, A. (2011). Taxus wallichiana, East Himalayan Yew. The IUCN Red List of Threatened Species.

Thomas, P., Li, N., & Christian, T. (2013). Taxus chinensis, Chinese Yew. The IUCN Red List of Threatened Species.

Weising, K., Atkinson, R. G., & Gardner, R. C. (1995). Genomic fingerprinting by microsatellite primed PCR: A critical evaluation. PCR Methods and Applications, 4(5), 249–255. https://doi.org/10.1101/gr.4.5.249

Widyatmoko, A., Lejo, E., Prasetyaningsih, A., & Rimbawanto, A. (2010). Keragaman genetik populasi Araucaria cunninghamii menggunakan penanda RAPD (Random Amplified Polymorphic DNA). Jurnal Pemuliaan Tanaman Hutan, 4(2), 63–77.

Williams, J. G. K., Kubelik, A. R., Livak, K. J., Rafalski, J. A., & Tingey, S. V. (1990). DNA polymorphisms amplified by arbitrary primers are useful as genetic markers. Nucleic Acids Research, 18(22), 6531–6535. https://doi.org/10.1093/nar/18.22.6531

Yang, Y., Christian, T., & Li, N. (2013). Taxus mairei, Maire’s Yew. The IUCN Red List of Threatened Species. Retrieved from http://dx.doi.org/10.2305/IUCN.UK.2013-1.RLTS.T191659A1991533.en

Zarek, M. (2009). RAPD analysis of genetic structure in four natural populations of Taxus baccata from Southern Poland. Acta Biologica Cracoviensia Series Botanica, 51, 67–75. Retrieved from http://www.ib.uj.edu.pl/abc/pdf/51_2/08_zarek.pdf

DOI: https://doi.org/10.20886/jpth.2021.15.2.95-104