DEVELOPMENT OF ARBUSCULAR MYCHORRHIZHAE AND GROWTH OF TEAK (Tectona grandis Linn. F.) SEEDLINGS INOCULATED WITH SPORES OF ARBUSCULAR MYCORRHIZAL FUNGI ORIGINATED FROM SOIL IN TEAK FOREST

Corryanti Corryanti; Joedoro Soedarsono; Bostang Radjagukguk; Siti Muslimah Widyastuti

doi:10.20886/jpth.2007.1.2.51-61

DEVELOPMENT OF ARBUSCULAR MYCHORRHIZHAE AND GROWTH OF TEAK (Tectona grandis Linn. F.) SEEDLINGS INOCULATED WITH SPORES OF ARBUSCULAR MYCORRHIZAL FUNGI ORIGINATED FROM SOIL IN TEAK FOREST

Corryanti Corryanti, Joedoro Soedarsono, Bostang Radjagukguk, Siti Muslimah Widyastuti

Abstract

The objective of the study was to determine the effect of arbuscular mycorrhizal fungi collected from soil of teak plantation forest on the growth of teak seedling related to mycorrhizal association development. The study utilized the complete randomized design with three levels of inoculant’s factor, consisting of control (uninoculated), inoculation with Gigaspara spores and inoculation with Glomus spores inoctilated, with ten replications. Spores isolated originated from soil of teakplantation forest in Tangen, Surakarta. The planting medium was a mixture soil sand at 1:1 (v/v) sterilized prior to be used. NPK mixed ferfilizer was applied at amount 0,0625 gr per seeadling mixed in to the planting medium. Measurements were conducted during a five month period. The data were analyzed using the Analysis ofVariance method and differences among treaments analyzed usfing the Duncan’sMultiple Range Test at the 5% ievel of significance. The results showed that inoculationwith the arbuscular mycorrhizail fungi increased teak seedling growth, with Gigaspora sp inoculation giving the highest growth. The uptakes of N, P, K and Ca were increased by both Gigaspora sp and Glomus sp inoculations. The increased seedling growth, was accompanied by large increases in infection percentage as well as sporulation. The highest infection and sporulation were found with Gigaspora sp inoculation. These results showed that in the soil ofteak plantation forest in Tangen of the grumusol type, relatively low NPK fertilization (0,0625 gr per seedling) and inoculadon with spores ofarbuscular mycorrhizal fungi improved teak seedling growth, inereased nutrient uptake, as well as improved mycorrhizal association development in teak seedlings.

Keywords

Gigaspora sp; Glomus sp; mycorrhizal association; seedling growth; teak

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References

Abbot, L.K., Robson, A.D. Jasper, D.A., Gazey, C., 1992. What is the role VA-mycorrrhizal hyphae in soil? In.: Read, D,I., Lewis, D.H., Fittter, A.H., Alexander, (Eds) Mycorrhizas n ecosystem. lnternational, University Press, Cambridge. CAB. p.:26- 36.

Ali, S.S., Gupta, N. dan Rahangdale, R., 1995. Ecology ofvesicular-arbuscular mycorrhizal fungi in tropiral forest of central India In. Biology and biotechnology of mycorrhizae. Biotrop special publication No. 56, SEAMEO BIOTROP, Bogor. p. 49-53.

Bagyaraj, 1994. Handbook applied mycology: Ecology of VAM.Marcel Decker, Ine New York. p. 1-34.

Brundrett M.C., Bougher N., Dell B, Grove T. dan Malajezuk N., 1996. Working with mycorrhizas forestry and agriculture. ACIAR Monograph 32, Canberra, Australia.

Brundrett M.C., Abbot L.K. dan Jasper D.A. 1999. Glomalean mycorrhizal fungi from tropical Australia. Mycorrhiza 9:305-314.

Chan, SK, Thai, L.H., and Abas, .G., 1981. Study of cassava response to phosphorus with and without mycorrhizal inochdation. Malaysian society of soi I science. P: 383- 393.

Clark., R.B.1997.. Arbuscular mycorrhizal adaptation, spore germination, root coloni-zation, and host plant growth and mineral acquisition at low pH. Plant and Soil 192: 15- 22.

Corryanti dan Rohayati. 1999. Studi Efektifitas jenis endomikorizapada pembibitan jati. Prosiding Seminar Mikoriza I, Bogor p. 154-161.

Corryanti, Maryadi F. dan Irmawati, 2001. Arbuscular mycorrhizas fungi under teak Seed Orchard. Poster presented on the Third international Conference on Mycorrhizas: Diversity and integration in Mycorrhizas 8- 13 July,Adelaide, South Australia.

Fitter, A.H. dan Merry-Weather J.W., 1992. Why are some plants more mycorrhizal than others? An ecological enquiry.in. D.J. Read, D H Lewis, A H Fitter, Alexander (eds) Mycorrhizas in Ecosystems. CAB International, pp: 26-36,

Hardjodarsono, 1977. Jati. Edisi kedua. Bagian Penerbit Yayasan Pernbina Fakultas Kehutanan Universitas Gadjah Mada. Yogyakarta.

Harley, I.L., 1994, The state of the art. Techniques for Mycorrhizal Research (Eds. Noris J,R., Read, D.J., Varma, A.K.). Academic Press London. 1-24.

Hawkins, H., Johansen, A., George, E., 2000. Uptake and fransport of organic and inorganic nitrogen bu arbuskular mycorrhizal fungi. Plant and Soil 226: 275-285.

Irianto, R.S.B., Sintoso, E., Corryanti, R. Prematuri, M. Turjarnan. E. Widyati, I.R. Sitepu, S. Santoso, 2001. Pengaruh penggunaan cendawan mikoriza arbuskula, pupuk dan media tumbuh terhadap pertumbuhan bibit jati (Tectona grandis L, F.). Kerjasama penelitian Pusbang SDH Perum Perhutani—Puslitbanghut dan K A. Bogor. (publikasi terbatas)

Jackson, M. L., 1967. Soil chemical analysis. Prentice-Hall of India Private Limited. New Dclhi.

Jeffries, P. dan Dodd, J.C.. 1991.The use of mycorrhiral inrx-ulant.s. in and agriculture. ln. Arora, D.K., Rai, B. Mukerji, Knudsen. G.R. (Eds), Handbook of applied mycology. Soil and Plants Volume 1 p.155-185,

Jones, Jr. J. Benton, Benyamin, W, Mi Els, I I,A., 1991. Plant analysis handbook. Micro-macro Publishing, Inc.

Mason, B.A., Musoko, M.O., Last, F.T., 1992. Short-term changes Vesicular-.Arbuscular Mycorrhizal Spore Populations Terminalia Plantations Cameroon. In: Read, D.J., .Lewis, D.H., Fitter, A.H., Alexander, I.J., Mycorrhizas in ecosysterns. C.A.B. International. University Press, Cambrihe. P.:261-267,

Moutoglis, P, and Widden, P., 1996. Vesicular-arbuscular mycorrhizal spore populations maple (Acer saccharum marsh. L,) forest. Mycorrhiza 6: 9 -97.

Nagahashi, G., Douds Jr, D.D.., Abney, G.D., 1996. Phosphorus amendment inhibits hyphal branching of the VAM fungus Gigaspora margarita directly and indirectly through its effect on root exudation, Mycorrhiza 6: 403-408.

O'Keefe, D.M, dan Sylvia, D.M. 1991, Mechanisms of the vesicular-arbuscular mycorrhizal plant-growth response. In. Arora, D.K., Rai, B., Mukerji, K.G, Knudsen, G.R. (Eds) Handbook of applied mycology. Soil and Plants Volume I. p.35- 54,

Rajan, S.K,. Reddy B.J.D. Bagyaraj, D.J., 2000. Screening arbuscular mycorrhizal fungi for their symbiotic efficiency Tectona grandis. Forest ecology and management 126 (2000) 91-95.

Setiadi, Y., 1999. Status penelithin pemanfaatan cendawan mikoriza arbuskula untuk rehabilitasi terdegraciasi. Dalam. Prosiding Seminar Nasional mikoriza I. Bogor 15-16 Nopember 1999.

Suraya, 2002. Uji kompatibilitas Cendawan mikoriza arbuskula pada beberapa klon jati kultur jaringan Pusbanghut (Juni 03): 11, (publikasi untuk kalangan terbatas)

Sylvia, D.M,, 2005. Mycorrhizal symbioses. In Sylvia, D.M., Fuhrmann, J. J, Hartel, P.G., Zuberer, D.A. (Eds), PrincipIes and applications of soil microbioIogy. Upper Saddle River, New Jersey. p. 263-282.

Tan, K. H., 1995. Soil sampling, preparation, and analysis. Marcel Dekker, Inc. New York.

Thorn, G., 1997. The fungi in soil. In. Modern Soil Mycrobiology, Elsas et al (cds). Marcel Dekker, New York - Basel, pp: 63- 127.

Timothy, C., Paulitz and Linderrnan, R.G. 1991, Mycorrhizal interactions with soil organisms, In Handbook of applied Mycology Vol. 1: Soil and Plants. Arora D, K., Rai, B, Mukerji, K.G„ Knudsen, G.R. (Eds). Marcel Dekker, Inc. New York-Basel. P: 77-130.

Tommerup l. C., 1994. Methods for the study of the population biologi of vesicular-arbuscular mycorrhizalfungi.In Techniques for mycorrhizal research. J.R. Norris, D.J. Read, A.K. Varma (eds). Acadernic Pres. Lonclon,

Van der Heijden, E.W., Kuyper, T.W., 2001.Does origin of mycorrhizalfungus or mycorrhizal plant influence effectiveness of the mycorrhizal symbiosis? Plant and Soil 230: 161-174.

DOI: https://doi.org/10.20886/jpth.2007.1.2.51-61